(12) United States Patent (10) Patent No.: US 7,849,632 B2

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US007849632B2

(12) United States Patent

(10) Patent No.:

Deppermann et al. (54)

US 7,849,632 B2

(45) Date of Patent:

AUTOMATED SEED SAMPLER AND METHODS OF SAMPLING, TESTING AND

*Dec. 14, 2010

4,696,308 A

9/1987 Meller et al.

4,827,776 A

5/1989 Gale et al.

BULKING SEEDS

(75)

Inventors: Kevin L. Deppermann, St. Charles, MO (US); Michael W. Petersen, Sauk City,

(Continued)

WI (US)

FOREIGN PATENT DOCUMENTS

(73) Assignee: Monsanto Technology LLC, St. Louis, MO (US) (*)

Notice:

CL

1035-03

5/2003

Subject to any disclaimer, the term of this patent is extended or adjusted under 35

USC 154(b) by 0 days.

(Continued)

This patent is subject to a terminal disclaimer.

OTHER PUBLICATIONS

Brumback, Jr., et a1., “Automating fatty acid analyses from seeds: from ?eld samples to data bases,” Lab. Inf. Manage, 21 (1993) pp.

(21) Appl.No.: 12/563,895

215-222.

(22) Filed:

Sep. 21, 2009

(Continued) (65)

Pnor Pubhcatlon Data US 2010/0086963 A1

Primary ExamineriFrancis T Palo

APR 8, 2010

(74) Attorney, Agent, or Firmilames E. Davis; Joseph A. Schaper; Harness, Dickey & Pierce, P.L.C.

Related US. Application Data

(63)

Continuation of application No. 11/213,432, ?led on Aug. 26, 2005, noW Pat. No. 7,591,101.

(60) 26 Provisional application No. 1_60/604,604, ?led on Aug. 2004 _ _ 1 f N 60/691 100 ,

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prov1s1ona

?led on Jun‘ 15, 2005'

Int CL A01C 1/00 (52) US. Cl.

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(51)

(58)

(2006.01) 47/581 SE

Field of Classi?cation Search

47/58.1 SE,

47/581 FV

See application ?le for complete search history. (56)

References Cited U.S. PATENT DOCUMENTS 3,852,914 A 4,278,183 A

12/1974 Levengood 7/1981 Billington

(57)

ABSTRACT

An automated system for sampling seeds generally includes an automated sampler operable to remove a tissue sample from a seed, a seed tray con?gured to receive from the auto

mated sampler the seed from Which the tissue sample is removed, and a sample tray con?gured to receive the tissue sample removed from the seed. A table supports the seed tray and the sample tray, and an index mechanism is operable to _

_

_ _

_

move the table to brmg the seed tray mto pos1t1on for recelv

ing from the automated sampler the seed from Which the tissue sample is removed, and to bring the sample tray into position for receiving the tissue sample removed from the seed.

20 Claims, 32 Drawing Sheets

US 7,849,632 B2 Page 2 US. PATENT DOCUMENTS

5,677,474 A 6,537,826 B1

10/1997 Rogers 3/2003 Horigane

6,646,264 B1

11/2003 Modiano et al.

6,809,819 B1

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6,947,144 B2

7,044,306 B2 7,367,155 B2 7,502,113 B2 7,591,101 B2 *

7,611,842 B2 7,703,238 B2

9/2009

Vinjamoori et al. Kim et al. Deppermann Kotyk et al. Deppermann et a1. Deppermann ........ .. 47/58.1 SE

11/2009 Deppermann et a1. 4/2010 Deppermann et a1.

2001/0024796 A1 2002/0144458 A1 2003/0148258 A1

9/2001 Selifonov et a1. 10/2002 Hunter et al. 8/2003 Kim et al.

2003/0188998 A1

10/2003 Deppermann

2004/0091888 A1 2004/0221335 A1

5/2004 Nishio et al. 11/2004 Shewmaker et a1.

2005/0154221 A1 2006/0042527 A1*

2006/ 0042528 2007/0207485 2008/0113367 2008/0131254 2008/0131924 2008/0317279

A1 A1 A1 A1 A1 A1

2010/0086963 A1*

L.)”, Molecular Breeding, vol. 11, No. 3, Apr. 2003, pp. 187-201. Gillaspie, Jr., Sensitive Method for Testing Peanut Seed Lots for Peanut Stripe and Peanut Mottle Viruses by Immunocapture-Reverse

Transcription-Polymerase Chain Reaction, Plant Disease, May 2000, pp. 559-561.

Guidetti Geri, Viability Myths, , 4 pages. Higley P M et al., “Evaluation of Seed Biopsy Methods for Nonde

structive Seed Health Testing”, Phytopathology, St. Paul, MN, US, vol. 79, No. 10, Jan. 1989, p. 1140.

J .P. Hazebroek, “Analysis of genetically modi?ed oils” Progress in Lipid Research 39 (2000) pp. 477-506. Jones D A L M Barber et al., “An analysis of seed development in Pisum sativum L. XVI. Assessing variation for fatty acid content by use of a non-destructive technique for single-seed analysis”, Plant

Breeding, vol. 114, No. 1, 1995, pp. 81-83. Karcz Jagna et al., “Structural and embryological studies of diploid

3/2006

Deppermann ............. .. 111/171

and tetraploid Arabidopsis thaliana (L.) Heynah”, Acta Biologica

Deppermann Deppermann et a1. Becker et a1. Cope et al. Cope et al. Deppermann et a1.

Cracoviensia Series Botanica, vol. 42, No. 2, 2000, pp. 113-124. Kotyk et al., High-Throughput Determination of Oil Content in Corn

3/ 2006 9/2007 5/2008 6/2008 6/2008 12/2008

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673-03 2190-05 100 48 643 A1 1151988 1471076 2229210 536785 1658858 WO 98/14046 WO 01/89288 W0 03/084847 W0 03/100381 WO 2004/063333 WO 2006/026466 WO 2006/026467

2/2004 5/2007 5/2001 5/1969 4/1977 5/2004 11/1976 6/1991 4/1998 11/2001 10/2003 12/2003 7/2004 3/2006 3/2006

OTHER PUBLICATIONS

Cabrera et al., Open Storage of Soybean Seed in Mississippi, Mis

sissippi Agricultural and Forestry Experiment Station, Sep. 2002.

. Callaway A. S. et al., “High-Throughput Transgene Copy Number Estimation by Competitive PCR”, Plant Molecular Biology Reporter, vol. 20, Sep. 2002, pp. 265-277. Chunwongse J ., et al., “Pre-germination genotyping screening using PCR ampli?cation of half-seeds”, Theoretical and Applied Genetics, Springer, Berlin, DE, vol. 86, No. 6, Jan. 1993, pp. 694-698. Dahmer et al., “A Rapid Screening Technique for Determining the Lipid Composition of Soybean Seeds”, Journal of the American Oil Chemists’ Society, Springer, Berlin, DE, vol. 66, Jan. 1989, pp. 543-549.

methionine content of transgenic narbon bean (J/lcia narbonensis

7/2005 Lysenko et al.

FOREIGN PATENT DOCUMENTS CL CL DE GB GB RU SU SU W0 W0 W0 W0 W0 W0 W0

Demidov Dimitri et al., “Additive effects of the feed-back insensitive bacterial aspartate kinase and the Brazil nut 2S albumin on the

Kernels Using Nuclear Magnetic Resonance Imaging, JAOCS, vol. 82, No. 12, 2005, pp. 855-862. Kramer et al., Transgenic Avidin Maize is Resistant to Storage Insect

Pests, Nature Biotechnology, vol. 18, Jun. 2000, pp. 670-674. Krisnangkura K. et al., “Continuous transmethylation of palm oil in an organic solvent”, Jaoch, vol. 69, 1992.

Krysan, Breakthrough Technologies, Ice-Cap. A High-Throughput Method for Capturing Plant Tissue Samples for Genotype Analysis, Plant Physiology, Jul. 2004 vol. 135, pp. 1162-1169. McCarthy, Paul L., et al., “Rapid identi?cation of transformed Wheat

using a half-seed PCR assay”, Biotechniques, vol. 31, No. 3, Mar. 2002, pp. 560-564.

Morrison, Sampling in Seed Health Testing, The American Phytopathology, 1999, 89: 1084-1087. Pioneer Hi-Bred International, Inc., DoWnloadable PhotosiLaser Assisted Seed Selection, http://WWW.pioneer.com/Web/site/portal/ menuiteam.b9e99dcb8e2cfd8ecfe6d10093a0/, printed as of Nov. 25, 2008, 4 pages. Schuster Ivan et al., “Correlation between high molecular Weight

gluten subunits composition and bread-making quality in Brazilian Wheat”, Brazilian Journal ofGenetics, vol. 20, No.4, Dec. 1997, pp. 667-671. Sedcole, J .R. “Number of plants necessary to recover a trait,” Crop

Sci. 17:667 (1977). Serial Extraction of Endosperm Drillings (SEED)iA Method for Detecting Transgenes and Proteins in Single Viable Maize Kernels, Varaporn Sangton, et al., Plant Molecular Biology Reporter 19: 151 158, Jun. 2001, International Society for Plant Molecular Biology. Smith et al., Genetic Purity and Testing Technologies for Seed Qual ity.'A CompanyPerspective, Seed Science Research, 1998, vol. 8, pp. 285-293.

Van Der Mey J A M et al., “Mass Screening for Alkaloid Content in

Lupinus-Albus”, Applied Plant Science, vol. 1, No. 2, 1987, pp. 80-82.

Von Post et al., A High-Throughput DNA Extraction Method for

Barley Seed, Euphytica 130: 255-260, 2003. * cited by examiner

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