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UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING
HLA-B27 TYPING RESULTS OF SAMPLES 1B09&10/2017 DESPATCHED ON 17th October 2017 Summary of Results Total tested Positive Negative NT/Equivocal % Positive % Negative Consensus
124 120 4 3 96.8% 3.2%
Donor HLA type Donor HLA type
125 1 124 2 0.8% 99.2%
1B09/2017 1B10/2017
Positive
Negative
1 3 4 7 8 9 10 11 12 14 15 17 19 20 21 22 23 24 25 26 28 29
Result 1B09/2017 Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive
Result 1B10/2017 Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative
Date received 18-Oct 18-Oct 18-Oct 20-Oct 19-Oct 18-Oct 19-Oct 18-Oct 23-Oct 18-Oct 18-Oct 18-Oct 20-Oct 18-Oct 18-Oct 18-Oct 18-Oct 19-Oct 23-Oct 18-Oct 19-Oct 18-Oct
Date tested 19-Oct 19-Oct 30-Oct 20-Oct 19-Oct 20-Oct 19-Oct 31-Oct 26-Oct 25-Oct 19-Oct 26-Oct 25-Oct 19-Oct 18-Oct 18-Oct 23-Oct 27-Oct 25-Oct 20-Oct 23-Oct 18-Oct
Serological Molecular Serological Molecular Molecular Molecular Molecular Serological & Mol Molecular Serological Serological Molecular Molecular Molecular Molecular Molecular Molecular Molecular
31 32
Positive NT
Negative NT
18-Oct 18-Oct
18-Oct 19-Oct
Serological Molecular
34 35 37 39 40 41 42
Positive Positive Positive Positive Positive Positive Positive
Negative Negative Negative Negative Negative Negative Negative
18-Oct 18-Oct 18-Oct 18-Oct 18-Oct 18-Oct 18-Oct
19-Oct 19-Oct 20-Oct 19-Oct 24-Oct 20-Oct
Molecular Molecular Molecular Molecular Serological Molecular Molecular
45 46
Positive Negative
Negative Negative
19-Oct 18-Oct
24-Oct 27-Oct
Molecular Serological
47 48
Positive Positive
Negative Negative
18-Oct 19-Oct
18-Oct 26-Oct
Serological Molecular
54 55 57 58 62 66 67 72 76
Positive Positive Positive Positive Positive Positive Positive Positive Equivocal
Negative Negative Negative Negative Negative Negative Negative Negative Negative
19-Oct 18-Oct 18-Oct 18-Oct 20-Oct 18-Oct 19-Oct 20-Oct 18-Oct
23-Oct 19-Oct 18-Oct 20-Oct 20-Oct 23-Oct 19-Oct 27-Oct 19-Oct
Molecular Molecular Serological Molecular Molecular Molecular Serological Molecular Serological
77 78 83 84 85 86 90 96 101 103 104 106 107 108 109 110 111 112 113 115 126 127 128 129 130 133 137 142 153 154 156 174 176 185
Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Negative Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive
Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Positive Negative Negative Negative Negative Negative Negative Negative
18-Oct 18-Oct 19-Oct 18-Oct 18-Oct 18-Oct 20-Oct 19-Oct 17-Oct 18-Oct 17-Oct 20-Oct 27-Oct 19-Oct 18-Oct 19-Oct 19-Oct 24-Oct 18-Oct 18-Oct 23-Oct 18-Oct 18-Oct 26-Oct 17-Oct 19-Oct 18-Oct 17-Oct 18-Oct 18-Oct 18-Oct 23-Oct 23-Oct 19-Oct
20-Oct 26-Oct 20-Oct 19-Oct 19-Oct 20-Oct 21-Oct 25-Oct 19-Oct 19-Oct 17-Oct 24-Oct 27-Oct 24-Oct 23-Oct 19-Oct 19-Oct 25-Oct 24-Oct 24-Oct 30-Oct 26-Oct 19-Oct 30-Oct 20-Oct 20-Oct 18-Oct 27-Oct 19-Oct 26-Oct 23-Oct 28-Oct 23-Oct 24-Oct
198 209 219 223 224 225 232 234 236 245 252 255 256 257 269
Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive
Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative
18-Oct 18-Oct 24-Oct 18-Oct 18-Oct 23-Oct 20-Oct 28-Oct 20-Oct 18-Oct 20-Oct 18-Oct 18-Oct 18-Oct
19-Oct 19-Oct 27-Oct 23-Oct 25-Oct 24-Oct 23-Oct 01-Nov 24-Oct 24-Oct 24-Oct 23-Oct 18-Oct 19-Oct
Serological Molecular
273 276 278 279 288 292 295 300 305 306 307 317 319 324 325 326 327 329 334 339 340 348 349 353 357 359 362 365 368 370 372 373 377 378
Positive Positive Positive Positive Positive Positive Positive NT Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive
Negative Negative Negative Negative Negative Negative Negative NT Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative
18-Oct 18-Oct 23-Oct 18-Oct 19-Oct 19-Oct 18-Oct
Lab No
HLA-A25, A29; B18, B27; Cw1, Cw12; DR1, DR15, DQ5, DQ6 HLA-A11, A24; B8, B13; Cw6, Cw7; DR17, DR7, DQ2, -
Method Molecular Serological Molecular
Primers and Probes Alta Bioscience
Commerical kit
Serological & Mol Serological & Mol Serological Molecular Serological Molecular Molecular Molecular Serological Molecular
One Lambda
Own design Own design Commerical kit Own design Own design
Detection system Gel
High Resolution Typing HLA-B*27 alleles 1B09/2017 1B10/2017
Comments
Fluorescence
Gel Gel Olerup
without hybridization probes Own design Commerical kit
Olerup
Own design Commerical kit Commerical kit
One Lambda Biofortuna
Gel Gel Gel Fluorescence Gel Gel Gel Gel Gel, BAG Healthcare reverse dot blot
Samples accidentally put in fridge for 2 days. As we test via Flow Cytometry, this may have invalidated the results. Thus we have not submitted our results (as advised). Own design Own design Own design
Gel Gel Gel
Own design
Gel 27:03/05/13/17/19/28/55/56/58/60/64N/70/ 72/74/80/82/84/87/88/90/93/94N/9699/104/110/111/117/121/123/124/128/132/ 133/135/140/141/143-146/150/155/159
Own design
Gel
27:05/49/90/116-162 1B09 falls 10 +/- of decision line. If this was a patient it would not be reported
Commerical kit
Invitrogen
Gel
Commerical kit
Hain
Fluorescence
Commerical kit Commerical kit Own design
Innotrain Olerup
Gel Gel Fluorescence
Commerical kit
Tib MOLBIOL
Fluorescence
27:05:23, 27:18/23/29/75/85/157 are not detected by this method
27:05:23, 27:18/23/29/75/85/157 are not detected by this method
Duraclone HLA-B27 method, result 09 falls within indeterminant range
Serological Molecular Commerical kit Serological Serological & Mol Molecular Molecular Commerical kit Serological Molecular Molecular Serological Serological Molecular Molecular Molecular Serological & Mol Serological Molecular Molecular Molecular Molecular Molecular Serological & Mol
PCR-SSP Kit Manufacturer
Olerup
Gel
Invitrogen
Gel
Sample 1B09/2017 equivocal, conformation positive exon 2 and exon 3
Commerical kit
Olerup
Gel
Commerical kit
Gel
Commerical kit
Gel
Commerical kit
One Lambda
Commerical kit Commerical kit
Olerup SSP Olerup
Own design
negativeexon 2 and exon 3
Fluorescence Gel Gel Fluorescence
Commerical kit
Olerup
Gel
Commerical kit
Olerup
Gel
Molecular Molecular Molecular Molecular Molecular Serological Molecular Molecular Molecular Serological Molecular
Commerical kit Commerical kit Commerical kit Commerical kit Own design
Invitrogen Biofortuna Olerup Olerup SSP
Gel Gel Gel Gel Fluorescence
Commerical kit
Olerup
Commerical kit Commerical kit
Olerup Gel Euroimmun, Euroarray Fluorescence
20-Oct 23-Oct 23-Oct 23-Oct 19-Oct 19-Oct 18-Oct
Molecular Molecular Molecular Serological Serological Serological Serological
Commerical kit Commerical kit
Olerup Linkage Bioscience
05-Oct 18-Oct 19-Oct 23-Oct 18-Oct 19-Oct 18-Oct 20-Oct 23-Oct 19-Oct 24-Oct 17-Oct 18-Oct 19-Oct
25-Oct 19-Oct 20-Oct 26-Oct 19-Oct 19-Oct 25-Oct 26-Oct 24-Oct 20-Oct 24-Oct 19-Oct 23-Oct 19-Oct
Molecular Molecular Molecular Molecular Molecular Serological Molecular Serological Molecular Molecular Serological Molecular Molecular Serological
18-Oct 10-Oct 18-Oct 18-Oct 18-Oct 19-Oct 21-Oct 19-Oct 19-Oct 25-Oct 24-Oct
24-Oct 17-Oct 25-Oct 26-Oct 20-Oct 26-Oct 23-Oct 24-Oct 20-Oct 25-Oct 24-Oct
Molecular Serological Molecular Molecular Serological Molecular Serological Serological Molecular Serological Serological
27:01-27:05:05, 27:05:10-27:05:15, 27:05:17-27:05:22, 27:05:24-27:05:32, 27:08/10/12-13/15-15/25/28/36-40/42/4445/47-69/71-74/79-80/82-84/86-90:03/92100/103-105/108-124/126/128/131135/137/139/141-149/151-152/155-156
Gel positive exon 2 and exon 3
Samples did not arrive at the laboratory
Commerical kit
Olerup
Gel
Commerical kit
Olerup
TapeStation
Commerical kit Commerical kit Commerical kit
Bionobis Bionobis AnDiaTec
Gel Fluorescence
Own design
Commerical kit Own design
Fluorescence
Appolon Bioteck
NEQ-115
Fluorescence Fluorescence
UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
In house HLA-B27 Samples tested at 6 days old
Issue 2 P. 1 of 2 Effective Date 10/10/16
UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING
HLA-B27 TYPING RESULTS OF SAMPLES 1B09&10/2017 DESPATCHED ON 17th October 2017 Lab No
Source of pos/neg control cells used (if applicable) 10 IH 17 20 Control Cells: B7 Homozygous Cell, B27/B7 Positive cell, B27/B7 Negative cell 21 27 Positive control: Crisp HLA-B27 positive control cells from Phoenix Flow Systems Negative control: ImmunoTrol cells from Beckman Coulter 31 CRISP Cells
Designation HLA-B27 145.2 B27 FITC B27-FITC, B27 and B7, Becton Dickinson
Monoclonal 1 Specificity HLA-B27 B27
Manufacturer BD One Lambda
HLA-B27-FITC One Lambda Beckman Coulter Duraclone
110 N/A 111 128
133 In house 137 324 334 365 Internal control in each Duraclone B27 tube (Bc) (beads)
Monoclonal 2 Specificity
Manufacturer BD BD
Designation HLA-B27 FD705 9E1010 HLA-B27
Monoclonal 3 Specificity
Manufacturer One Lambda
N/A
HLA-B27-FITC / HLA-B7PE Beckman Coulter
IOTest HLA-B27-FITC HLA-B7-PE, HLA-B27 and HLA-B7, Beckman Coulter
IgG2a-FITC, IgG1-PE, Isotype Control, Beckman Coulter 68 Positive Controls -Crisp HLA-B27 HLA B27-FITC/HLA B7Supplier- Phoenix Flow Systems PE, Beckman Coulter
76 HLA-B27 pos CRISP cells supplied by Enzo Life Sciences.
Designation CD3 SK-7 CD3 CD3 PE CD3 B27-FITC, B27, One Lambda
DuraClone B27, HLA-B27, Beckman Coulter
HLA B27-FITC, One Lambda
FD705
HLA-B27
Beckman Coulter
Anti HLA B27 FITC Anti HLA B27 FITC IOTEST HLA-B27-FITC, HLA-B27, Beckman coulter Anti CD27 HLA-ABCm3/BB71 Anti HLA B27 FITC HLA-B27 - FITC HLA-B27 (clone FD 705)
FD705-9E1E10 Clone GS145.2
One Lambda BD Biosciences
CD27 HLA-B-27HLA-B7 Anti HLA B27 FITC HLA-B27 PE
BC Immunotech Beckman Coulter Beckman Coulter Beckman Coulter
ABCm3
HLA-B27
Beckman Coulter
BB7.1
HLA-B7
Beckman Coulter
CD3 PE Clone SK7 IOTEST HLA-B7-PE, HLA-B7, Beckman coulter
BD Biosciences
Anti CD7 MPC-11 HLA-B7-PE HLA-B27-PE HLA-B27 (clone HLAABC-m3)
BC One Lambda
Anti CD3
T cells
BC
Beckman Coulter
CD3 (clone UCHT1)
PE-Dyomics 649
Beckman Coulter
CD7 HLA-B27 HLA-B7 HLA-B27 PE
CD45 PerCP-Cy5.5, Lymphocytes Gating, Becton Dickinson
NEQ-115 Issue 2 UK NEQAS for H&I is operated by Velindre NHS Trust, a UKAS proficiency testing provider No 8351. © Confidential report; no data may be published without permission from UK NEQAS for H&I
P. 2 of 2 Effective Date 10/10/16
