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UK NEQAS FOR H&I SCHEME 2A - CYTOTOXIC CROSSMATCHING PBL/T-CELL AND B-CELL CROSSMATCHING RESULTS WITHOUT DTT OF SAMPLE 2A04/2015 OS PC DESPATCHED ON 24TH MARCH 2015 HLA PHENOTYPE OF BLOOD DONOR: HLA-A1, A3; B8, B35; Cw4, Cw7; DR1, DR17; DQ2, DQ5 Summary of Results PBL/T-Cells
B-Cells
Total tested Positive Negative NT/Equivocal % Positive % Negative
29 13 16 3 44.8% 55.2%
30 1 29 3 3.3% 96.7%
29 3 26 4 10.3% 89.7%
30 5 25 3 16.7% 83.3%
23 23 0 1 100.0% 0.0%
23 0 23 1 0.0% 100.0%
23 4 19 1 17.4% 82.6%
22 5 17 2 22.7% 77.3%
Consensus
Not Assessed
Negative
Negative
Negative
Positive
Negative
Negative
Negative
DQ1
DQ3
A2, B17
A9, DR15
DQ1
DQ3
A2, B17
A9, DR15
Serum 1
Without DTT Serum 2 Serum 3
Serum 4
Serum 1
Without DTT Serum 2 Serum 3
Serum 4
Positive Positive Negative Positive Negative Negative Negative Negative Negative Negative NT Positive Positive Negative Negative Negative NT NT Negative Positive Negative Positive Positive Negative Positive Positive Positive Negative Positive Positive Negative Negative
Negative Positive Negative Negative Negative Negative Negative Negative Negative Negative Negative NT Negative Negative Negative Negative Negative NT NT Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative
Negative Positive Positive Negative Negative Negative Negative Negative Negative Negative Negative NT Positive Positive Negative Negative Negative NT NT Negative Negative Negative Negative Negative Positive Negative Negative Negative Negative Negative Negative Negative Negative
Positive
Negative
Negative
Positive
Positive
Negative
Positive
Positive
Positive Positive Positive Positive
Negative Negative Negative Negative
Negative Negative Negative Negative
Equivocal Negative Negative Negative
Positive Positive Positive Positive Positive NT Weak Positive Positive
Negative Negative Negative Negative Negative NT Negative Negative
Positive Positive Negative Negative Negative NT Negative Negative
Positive Positive Negative Negative Negative NT Negative Negative
Positive
Negative
Negative
Negative
Positive Positive Positive Positive Positive Positive Positive Positive Positive
Negative Negative Negative Negative Negative Negative Negative Negative Negative
Positive Negative Negative Negative Negative Negative Negative Negative Negative
Positive Negative Negative Negative Negative Negative Negative Negative Negative
HLA Antibody Specificity (Defined By CDC)
Lab No. 112 116 119 120 128 139 143 147 154 157 159 174 181 189 190 193 194 195 197 209 216 223 229 232 238 245 252 259 260 262 293 294 297
PBL/T Cell
B Cell
Assessment Assessment
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
NO NO
NO YES NO YES
NO YES YES YES YES
NO YES YES YES YES YES YES YES YES
NO YES NO
NO YES YES YES YES YES YES YES YES YES
Negative Negative Equivocal Negative Negative Negative Negative Negative Negative Negative Negative NT Positive Positive Negative Negative Negative NT NT Negative Negative Negative Negative Negative Positive Negative Negative Negative Negative Negative Negative Negative Negative
Poor cell viability
Page 1 of 4 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
No results returned Poor T cell viability
UK NEQAS FOR H&I SCHEME 2A - CYTOTOXIC CROSSMATCHING PBL/T-CELL AND B-CELL CROSSMATCHING RESULTS WITHOUT DTT OF SAMPLE 2A04/2015 OS PC DESPATCHED ON 24TH MARCH 2015
Lab No. 112 116 119 120 128 139 143 147 154 157 159 174 181 189 190 193 194 195 197 209 216 223 229 232 238 245 252 259 260 262 293 294 297
Serum 1 reaction strength
PBL/T-cell without DTT Serum 2 Serum 3 reaction reaction strength strength
Serum 4 reaction strength
6 6 6 1 4 1 1 1 1
-1 6 1 1 1 1 1 1 1
-2 1 2 1 1 1 1 1 1
-1 4 4 1 1 1 1 1 1
1 NT 6 6 5% 1 1
1 NT 1 1 5% 1 1
1 NT 4 6 5% 1 1
1 NT 4 6 5% 1 1
NT
NT
NT
NT
Serum 1 reaction strength
B-cell without DTT Serum 2 Serum 3 reaction reaction strength strength
Serum 4 reaction strength
Cell viability % PBL T-cells B-cells 90 90
NT 8
NT 1
NT 1
NT 2
8
1
4
8
8 8
1 1
1 1
2 1
8
1
1
1
8 6 95% 3 8
2 1 10% 1 1
4 6 10% 1 1
4 6 15% 1 1
95 80 80
100
83 92
100 80
8 6 1 90% 6+ 1 6 4-6
4 8+ 0 1
1 1
1 1 1
0 1
2 1
2 1 1-2
0 1
2 1
100
100
80 90 100 95 100 100
80 100 95 100 100
90 95 99 98
83 88 95 99 98
40 95
70 90
90
80
90 100
80 100 50 85 90 100 60 95 80
90 6
4 1 1
8 6 4-6
0 1
+++ 8 8+ 4 8
1
1 1 1
1
4 1 1-2
2
6 1 1
90 80 90 100 60 90 100
0 1
0 1
0 1
80
80 90 100 70 95 80
Page 2 of 4 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
UK NEQAS FOR H&I SCHEME 2A - CYTOTOXIC CROSSMATCHING PBL/T-CELL AND B-CELL CROSSMATCHING RESULTS WITH DTT OF SAMPLE 2A04/2015 OS PC DESPATCHED ON 24TH MARCH 2015 HLA PHENOTYPE OF BLOOD DONOR: HLA-A1, A3; B8, B35; Cw4, Cw7; DR1, DR17; DQ2, DQ5 Summary of Results PBL/T-Cells
B-Cells
Total tested Positive Negative NT/Equivocal % Positive % Negative
23 11 12 4 47.8% 52.2%
23 0 23 4 0.0% 100.0%
23 2 21 4 8.7% 91.3%
23 5 18 4 21.7% 78.3%
20 20 0 2 100.0% 0.0%
20 0 20 2 0.0% 100.0%
20 3 17 2 15.0% 85.0%
19 5 14 3 26.3% 73.7%
Consensus
Not Assessed
Negative
Negative
Negative
Positive
Negative
Negative
Not Assessed
DQ1
DQ3
A2, B17
A9, DR15
DQ1
DQ3
A2, B17
A9, DR15
Serum 1
With DTT Serum 2 Serum 3
Serum 4
Serum 1
With DTT Serum 2 Serum 3
Serum 4
Positive Positive Positive Negative Negative Negative NT
Negative Negative Negative Negative Negative Negative NT
Negative Negative Negative Negative Negative Negative NT
Negative Positive Positive Negative Negative Negative NT
Negative NT Positive Positive Negative Negative Negative NT NT Negative Positive Positive Negative Positive Positive Negative Positive Positive Negative Negative
Negative NT Negative Negative Negative Negative Negative NT NT Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative
Negative NT Positive Positive Negative Negative Negative NT NT Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative Negative
Negative NT Positive Positive Negative Negative Negative NT NT Negative Negative Negative Positive Negative Negative Negative Negative Negative Negative Negative
Positive Positive Positive NT Positive Positive
Negative Negative Negative NT Negative Negative
Negative Negative Negative NT Negative Negative
Positive Positive Equivocal NT Negative Negative
Positive Positive Positive Positive Positive NT Weak Positive Positive
Negative Negative Negative Negative Negative NT Negative Negative
Positive Positive Negative Negative Negative NT Negative Negative
Positive Positive Negative Negative Negative NT Negative Negative
Positive Positive Positive Positive Positive Positive Positive Positive
Negative Negative Negative Negative Negative Negative Negative Negative
Positive Negative Negative Negative Negative Negative Negative Negative
Positive Negative Negative Negative Negative Negative Negative Negative
HLA Antibody Specificity (Defined By CDC)
Lab No. 112 116 119 120 139 147 154 157 159 174 181 189 190 193 194 195 197 209 216 229 238 245 252 260 262 293 294 297
PBL/T Cell
B Cell
Assessment
Assessment
YES YES YES YES YES YES YES NO YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
NO NO
NO YES YES YES YES YES YES NO YES YES YES YES YES YES YES YES
NO NO YES YES YES YES YES YES YES YES
Low cell count
Poor cell viability
Page 3 of 4 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
No results returned Poor T cell viability
UK NEQAS FOR H&I SCHEME 2A - CYTOTOXIC CROSSMATCHING PBL/T-CELL AND B-CELL CROSSMATCHING RESULTS WITH DTT OF SAMPLE 2A04/2015 OS PC DESPATCHED ON 24TH MARCH 2015
Lab No. 112 116 119 120 139 147 154 157 159 174 181 189 190 193 194 195 197 209 216 229 238 245 252 260 262 293 294 297
Serum 1 reaction strength 6 6 4 1 1 1 NT
PBL/T-cell with DTT Serum 2 Serum 3 reaction reaction strength strength -1 1 1 1 1 1 NT
-2 1 1 1 1 1 NT
Serum 4 reaction strength -1 4 2 1 1 1 NT
Serum 1 reaction strength
B-cell with DTT Serum 2 Serum 3 reaction reaction strength strength
Serum 4 reaction strength
Cell viability % PBL T-cells B-cells 90 90 95
8 8 6 NT
1 1 1 NT
1 1 1 NT
2 6 2 NT
80
100 1 NT 6 6 5% 1 1
1 NT 1 1 5% 1 1
1 NT 4 6 5% 1 1
1 NT 4 6 5% 1 1
NT
NT
NT
NT
6
1
1
1
8 6 90% 4 8
2 1 5% 1 1
4 6 5% 1 1
4 6 5% 1 1
83 92
100 80
4 6 90% 1 6 4-6 4 8+ 0 1
1
2
2
1 1 1
1 1 1
4 1 1
0 1
0 1
0 1
8 6 4-6 +++ 8 8+ 6 8
1 1 1
4 1 1
6 1 1
90 90 90 100 60 100
0 1
0 1
2 1
80
100 80 100 95 100 100
100 80 100 95 100 100
90 95 99 98
83 88 95 99 98
40 95
70 90
90 100
80 100 50 90 100 60 95 80
90 100 70 95 80
Page 4 of 4 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
