(PDF) Black Currant Anthocyanins Attenuate

(PDF) Black Currant Anthocyanins Attenuate...

3 downloads 183 Views 1MB Size

Subscriber access provided by NEW YORK UNIV


Blackcurrant Anthocyanins Attenuate Weight Gain and Improve Glucose Metabolism in Diet-Induced Obese Mice with Intact, but Not Disrupted, Gut Microbiome Debora Esposito, Thanakorn Damsud, Mickey Wilson, Mary H. Grace, Renee Strauch, Xu Li, Mary Ann Lila, and Slavko Komarnytsky J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.5b00963 • Publication Date (Web): 12 Jun 2015 Downloaded from http://pubs.acs.org on June 17, 2015

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 38

Journal of Agricultural and Food Chemistry


Blackcurrant Anthocyanins Attenuate Weight Gain and Improve Glucose Metabolism in


Diet-Induced Obese Mice with Intact, but Not Disrupted, Gut Microbiome

3 4

Running title: Blackcurrant Anthocyanins and Gut Microbiome

5 6

Debora Esposito1,2, Thanakorn Damsud1§, Mickey Wilson1,2, Mary H. Grace1,2, Renee Strauch1,3,


Xu Li1,3, Mary Ann Lila1,2, Slavko Komarnytsky1,2*

8 9


Plants for Human Health Institute, North Carolina State University, North Carolina Research


Campus, 600 Laureate Way, Kannapolis, NC 28081




Dan Allen Drive, Raleigh, NC 27695




Place, Raleigh, NC 27695

Department of Food, Bioprocessing & Nutrition Sciences, North Carolina State University, 400

Department of Plant and Microbial Biology, North Carolina State University, 100 Derieux

15 16



Rajamangala University of Technology Srivijaya, 109 Thung Song, Nakhon Si Thammarat,


Thailand 80110

Current address: Program of Thai Traditional Medicine, Faculty of Science and Technology,

19 20

* Corresponding author


Phone: (704) 250-5459; Fax: (704) 250-5425; Email: [email protected]

22 23

Disclosure statement: None

1 ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry




Blackcurrant (Ribes nigrum L) is a rich source of anthocyanins, however the relationship


between their apparently limited bioavailability and significant protection against metabolic


pathologies is poorly understood. In this study, we examined gastrointestinal distribution of


blackcurrant anthocyanins and their phenolic acid metabolites in lean and diet-induced obese


mice with healthy and antibiotic-disrupted microbiome. Daily consumption of low or high fat


diet supplemented with 1% blackcurrant powdered extract (32% anthocyanins) for 8 weeks


reduced body weight gain and improved glucose metabolism only in mice with the intact gut


microbiome. Administration of antibiotic cocktail resulted in the 16-25 fold increase (P