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UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A08/2015 DESPATCHED ON 14TH JULY 2015 CONSENSUS HLA TYPE: HLA-A24, A31; B60, -; DR4, -; DQ8, Assessment Lab No. HLA- HLA- HLA- HLA- HLAA B Cw DR DQ 9 11 20 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 163 164 181 186 193 194 206 223 225 227 232 238 245 262 268 279 284 285 289 294 297 299 315 323
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES YES
HLA-A
A
B
24 24(9) 24 24 24 24 24 24 24 24 24 24 NT 24 24(9) 24 24 24 24 24 24 24 24 24 24 24 24(9) 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24
31 31(19) 31 31 31 31 31 31 31 31 31 31 NT 31 31(19) 31 31 31 31 31 31 31 31 31 31 31 31(19) 31 31 31 31 31 31 31 31 31 31 31 31 31 31 31 31
60 60(40) 60 60 60 60 60 60 60 60 60 60 NT 60 60(40) 60 60 60 60 60 60 60 60 60 60 60(40) 60 60 60 60 60 60 60 60 60 60 60 60 60 60 40 60
B
C
C
10 10 10 10
10
NT
NT 3
60(40) 3 3
NT 3
DR
3
DQ
4 4 4 4 4 4 4 4
8 8(3) 8 8 8 8 8 8
4 4 4 NT 4 4 4
8 8 8 NT 8 8 8
4 4 4 4 4 4 4 4
60
DR
NT 4
10
4
8 8 3 8 8 8 8 8
4
8
4 4
8 8
4
8
4 4
3 8
4 4
3
3 3
DQ
NT 8
8
Date received
Date tested
15-Jul 15-Jul 15-Jul 15-Jul 17-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 20-Jul 17-Jul 15-Jul 15-Jul 16-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 16-Jul 15-Jul 15-Jul 15-Jul 15-Jul 20-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 15-Jul 17-Jul 15-Jul 15-Jul 15-Jul 15-Jul 20-Jul
15-Jul 16-Jul 16-Jul 16-Jul 22-Jul 16-Jul 15-Jul 15-Jul 15-Jul 16-Jul 16-Jul 16-Jul 20-Jul 17-Jul 16-Jul 24-Jul 17-Jul 15-Jul 16-Jul 15-Jul 15-Jul 16-Jul 15-Jul 15-Jul 16-Jul 16-Jul 20-Jul 16-Jul 15-Jul 21-Jul 16-Jul 15-Jul 15-Jul 15-Jul 16-Jul 15-Jul 16-Jul 22-Jul 16-Jul 16-Jul 15-Jul 15-Jul 20-Jul
Comments
Low cell count and viability
Negative for DQ7. Cannot distinguish the DQ8 from DQ9. Molecular HR is DQB1*03:02
Page 1 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
UK NEQAS FOR H&I SCHEME 1A - HLA TYPING PHENOTYPING ASSESSMENT TYPING RESULTS OF SAMPLE 1A08/2015 DESPATCHED ON 14TH JULY 2015 CONSENSUS HLA TYPE: HLA-A24, A31; B60, -; DR4, -; DQ8, -
Lab No. 9 11 20 28 41 54 58 62 116 119 120 123 133 139 142 145 149 154 159 163 164 181 186 193 194 206 223 225 227 232 238 245 262 268 279 284 285 289 294 297 299 315 323
Supplemental DNA typing performed using: PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSP Luminex PCR-SSP RT-PCR PCR-SSO PCR-SSP/SBT
PCR-SSO PCR-SSO PCR-SSP PCR-SSO PCR-SSP PCR-SSP PCR-SSO PCR-SSP PCR-SSO PCR-SSO PCR-SSP/SSO PCR-SSO PCR-SSP/SBT PCR-SSP PCR-SSP PCR-SSP PCR-SSO PCR-SSO PCR-SSP PCR-SSO PCR-SSP/SSO
U
Viability % T B
95 100 90 5
95
95 95
90 0
99 95 99 90
95 70 99 90 100
5 90
90
100
100
99 100
99 100
90
90 88 99 99 90
100 88 95 99 100
99 100 100 95 100
98 80 95 100
90
98 100 98 100 90 95
60
100
PCR-SSP CDC PCR-SSO PCR-SSO PCR-SSP
95
90 80
PCR-SSO
80
95 90 95 50 80
Tray Manufacturer
Comments
One Lambda One Lambda One Lambda Innotrain, One Lambda One Lambda Innotrain, One Lambda One Lambda Biorad Biorad Ingen One Lambda One Lambda One Lambda Biorad Biorad, Innotrain Biotest One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda One Lambda Innotrain Innotrain One Lambda Biotest Ingen One Lambda One Lambda One Lambda Biorad, BAG One Lambda Biorad One Lambda One Lambda One Lambda Biorad, BAG One Lambda One Lambda
Very low cell viability
Page 2 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I
