Scheme 1B - 2015-07-08

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UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING HLA-B27 TYPING RESULTS OF SAMPLES 1B07&08/2015

DISPATCHED ON 21ST JULY 2015

Summary of Results Total tested Positive Negative NT/Equivocal % Positive % Negative

112 112 0 2 100.0% 0.0%

111 110 1 3 99.1% 0.9%

Consensus

Positive

Positive

Lab No

Result 1B07/2015

Result 1B08/2015

Date received

Date tested

Method

1 3 4 7 8 9 10 11 12 14 15 16 17 19 20 21 22 23 24 25 26 27 28 29 31 32 34 35 37 39 40 41 42 45 46 48 54 55 57 58 62 66 67 68 71 78 83 84 85 86 90 93 96 101 103 104 106 107 108 110 111 112 113 115 126 127 128 129 130 131 137 142 153 154 156 174 176 185 198 199 209 219 223 225 232 234 236 245 253 255 256 257 269 273 276 278 279 285 288 295 298 300 305 306 307 310 317 319 324 325 326 327 329 334

Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive

Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Negative Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Equivocal Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive Positive

22-Jul 22-Jul 22-Jul 22-Jul 22-Jul 22-Jul 22-Jul 20-Jul 22-Jul 23-Jul 22-Jul 22-Jul 24-Jul 22-Jul 22-Jul 22-Jul 22-Jul 23-Jul 22-Jul 27-Jul 22-Jul 22-Jul 22-Jul 22-Jul 22-Jul

31-Jul 22-Jul 05-Aug 23-Jul 22-Jul

Melting Curve Analysis Flow Cytometry PCR-SSP, Melting curve Flow Cytometry Flow Cytometry PCR-SSP/SSO Flow Cytometry PCR-SSO PCR-SSP PCR-SSO PCR-SSO PCR-SSP PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP/Melting curve PCR-SSP PCR-SSP PCR-SSP/SSO PCR-SSP Flow Cytometry PCR-SSO PCR-SSP/SSO Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSO PCR-SSP Flow Cytometry PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP PCR-SSP RT-PCR Flow Cytometry Flow Cytometry Flow Cytometry PCR-SSP Flow Cytometry PCR-SSP/Flow Cytometry RT-PCR PCR-SSP Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP Flow Cytometry Flow Cytometry Flow Cytometry Euro Array PCR-SSP PCR-SSP/Flow Cytometry Flow Cytometry PCR-SSP PCR-SSP PCR-SSP RT-PCR Cytotoxicity PCR-SSO/Flow Cytometry PCR-SSO Cytotoxicity/PRC-SSP Flow Cytometry Flow Cytometry RT-PCR Flow Cytometry PCR-SSP RT-PCR PCR-SSP Cytotoxicity PCR-SSP Flow Cytometry RT-PCR PCR-SSP RT-PCR PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSO RT-PCR RT-PCR Flow Cytometry PCR-SSP PCR-SSP PCR-SSP PCR-SSP MicroArray/SSP Cytotoxicity Cytotoxicity Flow Cytometry Flow Cytometry RT-PCR PCR-SSP PCR-SSP PCR-SSP PCR-SSP Flow Cytometry PCR-SSP

Donor HLA type Donor HLA type

22-Jul 22-Jul 02-Jul 23-Jul 21-Jul 22-Jul 22-Jul 22-Jul 23-Jul 23-Jul 22-Jul 22-Jul 22-Jul 23-Jul 22-Jul 22-Jul 22-Jul 22-Jul 22-Jul 22-Jul 23-Jul 22-Jul 22-Jul 22-Jul 21-Jul 22-Jul 23-Jul 23-Jul 23-Jul 27-Jul 24-Jul 24-Jul 22-Jul 25-Jul 23-Jul 22-Jul 29-Jul 22-Jul 24-Jul 03-Aug 22-Jul 23-Jul 22-Jul 22-Jul 23-Jul 22-Jul 24-Jul 27-Jul 27-Jul 23-Jul 22-Jul 21-Jul 22-Jul 22-Jul 28-Jul 23-Jul 29-Jul 23-Jul 22-Jul 27-Jul 23-Jul 22-Jul 22-Jul 27-Jul 22-Jul 22-Jul 29-Jul 22-Jul 22-Jul 22-Jul 22-Jul 28-Jul 23-Jul 15-Jul 22-Jul 22-Jul 24-Jul 29-Jul 22-Jul 23-Jul 22-Jul 22-Jul 27-Jul 22-Jul 14-Jul

22-Jul 28-Jul 07-Aug 27-Jul 29-Jul 24-Jul 03-Aug 24-Jul 23-Jul 23-Jul 22-Jul 27-Jul 05-Aug 24-Jul 23-Jul 24-Jul 27-Jul 30-Jul 22-Jul 24-Jul 28-Jul 05-Aug 28-Jul 22-Jul 31-Jul 27-Jul 23-Jul 28-Jul 24-Jul 28-Jul 03-Aug 23-Jul 30-Jul 06-Aug 23-Jul 22-Jul 22-Jul 06-Aug 23-Jul 27-Jul 22-Jul 23-Jul 24-Jul 23-Jul 24-Jul 29-Jul 23-Jul 24-Jul 23-Jul 24-Jul 29-Jul 28-Jul 24-Jul 22-Jul 04-Aug 23-Jul 27-Jul 04-Aug 23-Jul 31-Jul 04-Aug 22-Jul 24-Jul 22-Jul 23-Jul 27-Jul 27-Jul 28-Jul 31-Jul 27-Jul 28-Jul 22-Jul 24-Jul 27-Jul 06-Aug 31-Jul 31-Jul 28-Jul 04-Aug 28-Jul 27-Jul 06-Aug 30-Jul 22-Jul 22-Jul 28-Jul 24-Jul 02-Aug 03-Aug 23-Jul 23-Jul 22-Jul 24-Jul 30-Jul 24-Jul 24-Jul 23-Jul 28-Jul 27-Jul 05-Aug 23-Jul 23-Jul 22-Jul 22-Jul 29-Jul 06-Aug 15-Jul

1B07/2015 1B08/2015

HLA-A2, A3; B8, B27; Cw2, Cw7; DR4, -; DQ8,HLA-A1, A2; B8, B27; Cw1, Cw7; DR1, DR17; DQ2, DQ5

High Resolution Typing HLA-B*27 alleles 1B07/2015 1B08/2015

B*27:05/47/116/117

Comments

B*27:05/47/116/117

Sample 1B07/2015 would be referred to a specialist referral lab for confirmation

Sample 1B08/2015 - Negative by Duraclone, positive by One Lambda

Flow Cytometry PCR-SSP Flow Cytometry PCR-SSP PCR-SSO Flow Cytometry

Page 1 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

3 7 8 10 20 21 27 31 32 40 46 57 67 68

Monoclonal 1 Designation

HLA-B27 FITC HLA-B27 Duraclone B27 HLA-B27 145.2 B27-FITC Duraclone B27 Duraclone B27 MSIgG1 RDI/MsIgG2a FITC PF/FITC HLA-B27 PE CD3 IgG2a PED HLA-B27/B7 71 PEDy0649 - UHCT1 83 HLA-B27 84 B7/B27 90 HLA-B27 93 FITC 103 Iotest HLA-B27 FITC 104 Anti-HLA-B27 106 HLA-B27 110 Anti-HLA-B27 FITC 111 Anti-HLA-B27 FITC 128 Iotest HLA-B27 FITC 131 Iotest HLA-B27 FITC 137 HLA-ABC m3/BB7.1 153 HLA-B27 FITC 198 HLA-B27 testing 236 HLA-B27 FITC 256 Anti-HLA-B27 288 Anti-HLA-B27 295 Anti-HLA-B27 FITC 310 Iotest HLA-B27 FITC/B7 PE 324 HLA-B27 FITC 326 Iotest HLA-B27 FITC/B7 PE 334 HLA-B27 FITC

Lab No

DISPATCHED ON 21ST JULY 2015

CD3 B27 HLA-B27-FITC/HLA-B7-PE B7/B27 IgG/IgG2

CD3 SK3 B27-FITC Iotest HLA-B27 FITC/B7 PE PE/FITC CD3-PE-DY649 Anti-HLA-B27 IgG2b PE HLA-B27 PE-FD705 + ABCm3 PE PE B27 HLA-B7 HLA-B7/B27 Clone SK7 HLA-B7 HLA-B7 HLA-B27 IgG2a-IgG1 HLA-B27 CD3 PE Clone SK7 HLA-B7 HLA-B7 HLA-B27

HLA-B7 CD3 Duraclone Iotest HLA-B7 PE HLA-B27 CD3 PE Iotest HLA-B7 PE Iotest HLA-B7 PE MPC-11 HLA-B27-FITC/B7-PE HLA-B27 control Anti-CD3 Anti-CD3 Anti-CD3 PE Iotest HLA-B27 FITC/B7 PE HLA-B7 PE MoAb - B27 FITC HLA-B7 PE

FITC HLA-B27 B27 HLA-B27-FITC HLA B27

Clone SK7 PE

Monoclonal 2 Specificity

CD3 PE CD3

Monoclonal 2 Designation

BD BD BD Beckman Coulter Beckman Coulter One Lambda Beckman Coulter

BD Beckman Coulter Beckman Coulter One Lambda Immunotech Beckman Coulter

Immunotech

Beckman Coulter Beckman Coulter Beckman Coulter

Beckman Coulter

Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter One Lambda Beckman Coulter

BD One Lambda Beckman Coulter

BD BD

Monoclonal 2 Manufacturer

PE

HLA-B7

HLA-B27 cross

Anti-HLA-B7

Lymphocytes gating

Clone BB7.1

CD45 PerCp-Cy5.5

B7

PC5

HLA B7

Unlabelled BB7.1 CD3

CF3 B27

HLA-B27-FITC

HLA-B27 FITC

IgG1K PE

HLA-B27

Monoclonal 3 Specificity

FD705 9E1

Monoclonal 3 Designation

Page 2 of 2 UK NEQAS for H&I is provided from the Welsh Blood Service which is a division of Velindre NHS Trust © Copyright Notice: Reports are confidential, and no data may be published without permission from UK NEQAS for H&I

Beckman Coulter Beckman Coulter Beckman Coulter One Lambda Beckman Coulter BD One Lambda One Lambda BD Beckman Coulter Beckman Coulter Immunotech One Lambda BD Beckman Coulter BD BD BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter

FITC B7/B27 R-PE B27 HLA-B27 FITC/CD3/PE HLA-B27 FD705-9E1E10 Clone GS145.2 HLA-B27 HLA-B27 HLA-B27/B7 IgG2b FD705 HLA-B27 +CD3 HLA-ABC-m3 HLA-B27 FITC Clone GS145.2 HLA-B27 HLA-B27 HLA-B27

PE HLA-B27 CD3 HLA-B27-FITC/B7-PE CD3

BD BD Beckman Coulter BD BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter BD Beckman Coulter Beckman Coulter Beckman Coulter Beckman Coulter

Monoclonal 1 Manufacturer

Clone GS145.2 FITC CD3, HLA-B27, HLA-B7 HLA-B27 B27 CD3, HLA-B27, HLA-B7 CD3, HLA-B27, HLA-B7 Isotype control B7/B27

Monoclonal 1 Specificity

HLA-B27 TYPING RESULTS OF SAMPLES 1B07&08/2015

UK NEQAS FOR H&I SCHEME 1B - HLA-B27 TESTING

Beckman Coulter

Beckman Coulter

BD

Beckman Coulter

Beckman Coulter

Beckman Coulter

Beckman Coulter Beckman Coulter

One Lambda

One Lambda

Monoclonal 3 Manufacturer